positive markers  (Proteintech)

Journal: NPJ Regenerative Medicine

Article Title: Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration

doi: 10.1038/s41536-025-00444-9

Figure Lengend Snippet: A Schematic illustration of the ASCs isolation procedure from deer antler tissue using mechanical dissection and the collagenase digestion method. Created in BioRender. Yuhao, S. (2025) https://BioRender.com/m5easfx . B Representative phase-contrast microscopy image showing the typical spindle-shaped morphology of cultured ASCs at passage 3. Scale bar: 100 μm. C Immunofluorescence analysis of ASC surface markers. ASCs were positive for CD73 and CD90 (green), while negative for CD34 and CD45. Nuclei were counterstained with DAPI (blue). Merged images show co-localization. Scale bar: 100 μm. D – F Multi-lineage differentiation potential of ASCs. D Oil Red O staining showing lipid droplets after 14 days of adipogenic induction. Scale bars: 100 μm. E Alizarin Red S staining demonstrating calcium deposition after 21 days of osteogenic differentiation. Scale bars: 500 μm. F Alcian Blue staining revealing proteoglycan synthesis after 14 days of chondrogenic induction. Scale bars: 100 μm. G Western blot analysis confirming the expression of exosomal markers (CD63, TSG101, and CD9) in isolated ASC-Exos compared to ASCs and culture supernatant. H Representative transmission electron microscopy (TEM) image showing the typical cup-shaped morphology of ASC-Exos with characteristic double-membrane structure. Scale bars: 100 nm. I Nanoparticle tracking analysis (NTA) reveals the size distribution of ASC-Exos with an average diameter of 86.4 nm. J Scatter plot from flow cytometry analysis demonstrating the uniform distribution and homogeneity of the isolated ASC-Exos population.

Article Snippet: For flow cytometric analysis, cells were dissociated using trypsin without EDTA, washed with PBS, and incubated with surface marker antibodies at room temperature for 1 h. The panel included positive markers CD73 (bs-4834R, Bioss, China) and CD90 (bs-0778R, Bioss, China), and negative hematopoietic markers CD45 (bs-4819R, Bioss, China) and CD34 (bs-0646R, Bioss, China), following the manufacturer’s recommended antibody concentrations.

Techniques: Isolation, Dissection, Microscopy, Cell Culture, Immunofluorescence, Staining, Western Blot, Expressing, Transmission Assay, Electron Microscopy, Membrane, Flow Cytometry

Journal: Journal of Extracellular Vesicles

Article Title: Epidermal Stem Cell‐Derived Extracellular Vesicles Induce Fibroblasts Mesenchymal‐Epidermal Transition to Alleviate Hypertrophic Scar Formation via miR‐200s Inhibition of ZEB1 and 2

doi: 10.1002/jev2.70160

Figure Lengend Snippet: Isolation and characterization of the ESC‐EVs and FB‐EVs. (a) Schematic diagram of cell separation, culture and the EVs isolation. (b) Calcein and PI stains showed the high proliferative capacity of ESCs, FBs and HSFs. Scale bar, 100 µm. (c) Flow cytometry analysis of ESCs markers (cd49f and Krt15) and FBs markers (Pan‐Keratin and Vimentin). (d) Transmission electron microscopy (TEM) images of ESC‐EVs and FB‐EVs. Scale bar, 500 nm (down) and 200 nm (up). (e) Nanoparticle tracking analysis of ESC‐EVs and FB‐EVs from ZetaView. (f) Endosomal (Alix, TSG101) and tetraspanin (CD81, CD63) expression in FBs, ESCs, FB‐EVs and ESC‐EVs. GAPDH was used as a negative EVs marker.

Article Snippet: Western blotting was performed to detect positive markers Alix (1:1000, RGAB100‐50, Rengenbio), CD9 (1:1000, ab92726, Abcam), CD81 (1:1000, RGAB105‐50, Rengenbio), CD63 (1:1000, ab134045, Abcam), TSG101 (1:1000, ab125011, Abcam) and negative markers GAPDH (1:50000, 60004‐1‐lg, Proteintech) of EVs.

Techniques: Isolation, Flow Cytometry, Transmission Assay, Electron Microscopy, Expressing, Marker

Journal: Frontiers in Neurology

Article Title: Head position control strategies in progressive Supranuclear Palsy versus Idiopathic Parkinson’s Disease during dynamic-on-static platform tilt

doi: 10.3389/fneur.2024.1477493

Figure Lengend Snippet: (a) 3D ultrasound position markers (Zebris system) placement viewed from behind in the perspective of the ultrasound receptacle. Head (1,2,3 in red), upper chest “trunk” (4,5,6 in blue), hip (7 in green), and lateral femoral epicondyles (8,9 in yellow). (b) Mean Amplitudes of angular excursions for aforementioned body segments among healthy controls CTR, IPD, and PSP; the statistical mean is derived from the entirety of datasets from angular displacement conditions (0.5°/1°) and for eyes open and eyes closed (EO/EC).

Article Snippet: Zebris 3D real time ultrasound position markers were placed on the subjects, pointing backwards to define head (3 markers, one 5 cm over the vertex, the other two lateral to the ears positioned 4 cm away from the side of the skull and in line with the upper attachment point of the auricle), upper trunk (3 markers), hip (1 marker) and knee (1 marker 10 cm above each knee) motion and position during the experiments (placement see ).

Techniques: Derivative Assay